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Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine

Jianqing PAN PhD, Qin ZHANG MD, Daowen WANG MD, PhD,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 390-395 doi: 10.1007/s11684-009-0087-5

Abstract: We constructed a eukaryotic expression plasmid encoding Epstein-Barr virus latent membrane protein 2(EBV, LMP2) and evaluated its effects on humoral immunity.The serum antibody against LMP2 was detected with enzyme-linked immunosorbent assay (ELISA).The serum titer of IgG antibody against LMP2 epitope in the mice immunized with the DNA vaccine encodingLMP2 was up to 1∶4000.

Keywords： Epstein-Barr virus     latent membrane protein 2     nasopharyngeal carcinoma     humoral immunity    

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Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0855-9

Abstract: pH values of the BSA solution significantly impact the process of membrane fouling.Dramatic flux decline is caused by membrane–BSA adhesion force at start of filtration.XDLVO theory shows the polar or Lewis acid–base interaction plays a major role in membrane fouling.membrane–BSA, which illustrated that the more hydrophilic the membrane was, the better antifouling abilitymembrane fouling by changing the AB component of free energy.

Keywords： PVDF membrane     Membrane fouling     Adhesion force     Protein     Interfacial free energy    

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Protein phosphatase 2A, a key player in Alzheimer’s disease

Rong LIU, Qing TIAN

Frontiers of Medicine 2009, Volume 3, Issue 1,   Pages 8-12 doi: 10.1007/s11684-009-0017-6

Abstract: Protein phosphatase 2A (PP2A) is the predominant serine/threonine phosphatase in eukaryotic cells.is suggested to be involved in neurofibrillary tangle (NFT) formation, disturbed amyloid precursor proteinBased on our research and other previous findings, decreased PP2Ac level, decreased PP2A holoenzyme composition, increased level of PP2A inhibitors, increased PP2Ac Leu309 demethylation and Tyr307 phosphorylationFurther studies are required to disclose the role of PP2A in Alzheimer’s disease.

Keywords： protein phosphatase 2A     Alzheimer’s disease     holoenzyme composition     protein phosphatase 2A inhibitors     Leu309    

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Dual faces of SH2-containing protein-tyrosine phosphatase

Shuangwei Li, Diane DiFang Hsu, Hongyang Wang, Gen-Sheng Feng

Frontiers of Medicine 2012, Volume 6, Issue 3,   Pages 275-279 doi: 10.1007/s11684-012-0216-4

Abstract:

PTPN11, which encodes tyrosine phosphatase Shp2, is a critical gene mediating cellular responses toHowever, most recent experimental data suggest PTPN11/Shp2 acting as a tumor suppressor in hepatocarcinogenesisThis review focuses on the tumor-promoting or suppressing roles of the gene PTPN11/Shp2 in

Keywords： PTPN11/Shp2     leukemia     hepatocellular carcinoma     mutation    

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Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Frontiers of Medicine 2008, Volume 2, Issue 2,   Pages 166-170 doi: 10.1007/s11684-008-0030-1

Abstract: This study aims to demonstrate that blocking the receptor-interacting protein2 (Rip2) expression canconcentration was assayed with ELISA and high-mobility group box 1 protein (HMGB1) level with semi-quantitativeRip2 siRNA plasmid could block the mRNA and protein expression of Rip2 and promote cell proliferationBlocking Rip2 could attenuate LPS-induced TNF-? and HMGB1 production.The results demonstrate that Rip2 siRNA plasmid can block the expression of Rip2, decrease the production
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Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 2,   Pages 327-335 doi: 10.1007/s11783-014-0763-9

Abstract: the processes of ultra-sonication and acid precipitation, and then the humic acid was recovered via membraneThe acidic solution (pH 2) could be recycled and used more than 10 times during the co-recovery processes

Keywords： sewage sludge     co-recovery     proteins     humic acid     recycling     biodegradation rate    

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Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 235-238 doi: 10.1007/s11684-008-0044-8

Abstract: this study is to investigate the effect of tyrosine kinase Src on Tyrosine 307(Y307) phosphorylation, proteinphosphatase 2A (PP2A) activity, and on tau phosphorylation.of Src protein, and the phosphorylation levels of PP2A Y307 and tau at different sites, as well as PP2ATwelve hours after siRNA transfection, the protein level of Src was dramatically decreased, with decreasedHowever, the total PP2A protein level was also decreased, together with a decreased PP2A activity.

Keywords： hyperphosphorylation     PP2A activity     cellular regulation     siRNA     siRNA transfection    

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Encapsulation of polyethylene glycol in cellulose-based porous capsules for latent heat storage and light-to-thermal

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 8,   Pages 1038-1050 doi: 10.1007/s11705-022-2279-3

Abstract: Phase change materials are potential candidates for the application of latent heat storage.cellulose-based composite phase change materials exhibited high thermal energy storage ability with the latent

Keywords： cellulose     polyelectrolytes     phase change materials     thermal energy storage     light-to-thermal conversion    

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Annexin A2-S100A10 heterotetramer is upregulated by PML/RARα fusion protein and promotes plasminogen-dependent

Dan Huang, Yan Yang, Jian Sun, Xiaorong Dong, Jiao Wang, Hongchen Liu, Chengquan Lu, Xueyu Chen, Jing Shao, Jinsong Yan

Frontiers of Medicine 2017, Volume 11, Issue 3,   Pages 410-422 doi: 10.1007/s11684-017-0527-6

Abstract: protein causes lethal hyperfibrinolysis in acute promyelocytic leukemia (APL), but the mechanism isacid receptor a (PML/RARα) fusion protein, this work was performed to determine the transcriptionZinc-induced U937/PR9 cells expressed PML/RARα fusion protein, and resultant increases in ANXA2The transactivation of ANXA2 promoter by PML/RARα fusion protein was 3.29±0.13 fold higherThus, PML/RARα fusion protein transactivated the ANXA2 promoter to upregulate ANXA2 and accumulate

Keywords： annexin A2-S100A10 heterotetramer     PML/RARα fusion protein     plasmin     cell invasion     acute promyelocytic leukemia    

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Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation of membrane

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 1, doi: 10.1007/s11783-019-1188-2

Abstract: Analyze functional regulation of membrane proteins inthe transmembrane transport.mechanisms behind the microbial breakdown of polycyclic aromatic hydrocarbons (PAHs), including the role of membraneexplains the adsorption, uptake and transmembrane transport of PAHs by bacteria, the regulation of membraneprotein function during the transmembrane transport.Finally, recognize the microscopic mechanism of PAHs biodegradation in terms of cell and membrane proteins

Keywords： hydrocarbons     Transmembrane transport     Adsorption and uptake of hydrocarbons     Proteomics     Functional regulation of membraneprotein     Single cell analysis technology    

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VALORIZATION OF BIOGAS THROUGH SIMULTANEOUS CO AND HS REMOVAL BY RENEWABLE AQUEOUS AMMONIA SOLUTION IN MEMBRANE

Frontiers of Agricultural Science and Engineering 2023, Volume 10, Issue 3,   Pages 468-478 doi: 10.15302/J-FASE-2022473

Abstract:

● Simultaneous H2S and CO2 removal from biogas

Keywords： biomethane     biogas purification     CO2 removal     H2S removal     membrane absorption    

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Beyond bag of latent topics: spatial pyramid matching for scene category recognition

Fu-xiang LU,Jun HUANG

Frontiers of Information Technology & Electronic Engineering 2015, Volume 16, Issue 10,   Pages 817-828 doi: 10.1631/FITEE.1500070

Abstract: The proposed feature introduces spatial information among the latent topics by means of spatial pyramid, while the latent topics are obtained by using probabilistic latent semantic analysis (pLSA) based on

Keywords： Scene category recognition     Probabilistic latent semantic analysis     Bag-of-words     Adaptive boosting    

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Inhibition of protein kinase B by Palmitate in the insulin signaling of HepG2 cells and the preventive

XIA Yanzhi, WAN Xuedong, DUAN Qiuhong, HE Shansu, WANG Ximing

Frontiers of Medicine 2007, Volume 1, Issue 2,   Pages 200-206 doi: 10.1007/s11684-007-0038-y

Abstract: of free fatty acids (FFAs) may contribute to insulin resistance (IR) that is characteristic of type 2

Keywords： palmitate     characteristic     study     plasma     resistance    

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Diagnostic criteria of latent autoimmune diabetes in adults (LADA): a review and reflection

Yu Liao, Yufei Xiang, Zhiguang Zhou

Frontiers of Medicine 2012, Volume 6, Issue 3,   Pages 243-247 doi: 10.1007/s11684-012-0201-y

Abstract: Latent autoimmune diabetes in adults (LADA) is a subtype of autoimmune diabetes.Here, we aim to review and discuss the diagnostic criteria of latent autoimmune diabetes in adults.

Keywords： LADA     diagnostic criteria     autoantibodies     insulin independence     age of diagnosis    

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Antibody Therapies Targeting Complex Membrane Proteins Review

Georgina To´a Salazar, Ziyi Huang, Ningyan Zhang, Xue-Guang Zhang, Zhiqiang An,

Engineering 2021, Volume 7, Issue 11,   Pages 1541-1551 doi: 10.1016/j.eng.2020.11.013

Abstract:

In analyses of protein families that may serve as drug targets, membrane-associated G-protein-coupledHowever, various challenges put such membrane proteins among key groups of underutilized opportunitiesprotein, as well as adaptability through engineering into various antibody formats.However, the ease of raising and isolating specific, effective antibodies targeting membrane proteinsand discusses the preparation of membrane protein antigens and antibody generation, illustrated by select

Keywords： Antibody therapy     Complex membrane protein     Ion channels     Transporters     Membrane-bound enzymes     GPCRs    

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Title Author Date Type Operation

Construction and humoral immune response of Epstein-Barr virus latent membrane protein 2 DNA vaccine

Jianqing PAN PhD, Qin ZHANG MD, Daowen WANG MD, PhD,

Journal Article

Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

Journal Article

Protein phosphatase 2A, a key player in Alzheimer’s disease

Rong LIU, Qing TIAN

Journal Article

Dual faces of SH2-containing protein-tyrosine phosphatase

Shuangwei Li, Diane DiFang Hsu, Hongyang Wang, Gen-Sheng Feng

Journal Article

Blockage of receptor-interacting protein 2 expression by small interfering RNA in murine macrophages

LIU Hongchun, CAO Zhongwei, JIN Jianjun, WANG Jiyao

Journal Article

Application of ultra-sonication, acid precipitation and membrane filtration for co-recovery of protein

Liangliang WEI,Kun WANG,Xiangjuan KONG,Guangyi LIU,Shuang CUI,Qingliang ZHAO,Fuyi CUI

Journal Article

Effect of inhibiting tyrosine kinase Src expression on protein phosphatase 2A and tau phosphorylation

LIU Rong, ZENG Ji, ZHOU Xinwen, WANG Jianzhi, PEI Jinjing

Journal Article

Encapsulation of polyethylene glycol in cellulose-based porous capsules for latent heat storage and light-to-thermal

Journal Article

Annexin A2-S100A10 heterotetramer is upregulated by PML/RARα fusion protein and promotes plasminogen-dependent

Dan Huang, Yan Yang, Jian Sun, Xiaorong Dong, Jiao Wang, Hongchen Liu, Chengquan Lu, Xueyu Chen, Jing Shao, Jinsong Yan

Journal Article

Transmembrane transport of polycyclic aromatic hydrocarbons by bacteria and functional regulation of membrane

Hongqi Wang, Ruhan Jiang, Dekang Kong, Zili Liu, Xiaoxiong Wu, Jie Xu, Yi Li

Journal Article

VALORIZATION OF BIOGAS THROUGH SIMULTANEOUS CO AND HS REMOVAL BY RENEWABLE AQUEOUS AMMONIA SOLUTION IN MEMBRANE

Journal Article

Beyond bag of latent topics: spatial pyramid matching for scene category recognition

Fu-xiang LU,Jun HUANG

Journal Article

Inhibition of protein kinase B by Palmitate in the insulin signaling of HepG2 cells and the preventive

XIA Yanzhi, WAN Xuedong, DUAN Qiuhong, HE Shansu, WANG Ximing

Journal Article

Diagnostic criteria of latent autoimmune diabetes in adults (LADA): a review and reflection

Yu Liao, Yufei Xiang, Zhiguang Zhou

Journal Article

Antibody Therapies Targeting Complex Membrane Proteins

Georgina To´a Salazar, Ziyi Huang, Ningyan Zhang, Xue-Guang Zhang, Zhiqiang An,

Journal Article